Emergence of Hypervirulent Ceftazidime/Avibactam-Resistant Klebsiella pneumoniae Isolates in a Chinese Tertiary Hospital

Dan Li; Wenjian Liao; Hai-Hua Huang; Fang-Ling Du; Dan-Dan Wei; Yan-Fang Mei; Dan Long; La-Gen Wan; Yang Liu; Wei Zhang

doi:10.2147/IDR.S257477

Emergence of Hypervirulent Ceftazidime/Avibactam-Resistant Klebsiella pneumoniae Isolates in a Chinese Tertiary Hospital

Infect Drug Resist. 2020 Aug 3:13:2673-2680. doi: 10.2147/IDR.S257477. eCollection 2020.

Authors

Dan Li^#^{1

2}, Wenjian Liao^#^{1

2}, Hai-Hua Huang³, Fang-Ling Du⁴, Dan-Dan Wei⁴, Yan-Fang Mei⁴, Dan Long⁴, La-Gen Wan⁴, Yang Liu⁴, Wei Zhang¹

Affiliations

¹ Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang 330006, People's Republic of China.
² The First Clinical Medical College of Nanchang University, Nanchang University, Nanchang 330006, People's Republic of China.
³ Department of Endocrinology and Metabolism, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang 330006, People's Republic of China.
⁴ Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang 330006, People's Republic of China.

^# Contributed equally.

Abstract

Introduction: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) is increasingly reported worldwide, but ceftazidime/avibactam (CAZ/AVI)-resistant hvKP isolates have rarely been observed. We attempted to characterize them in clinical CRKP isolates collected from a university hospital in China from March 2016 to March 2018.

Methods: All isolates were analyzed by antimicrobial susceptibility testing, molecular detection of antibiotic resistance determinants, multilocus sequence typing (MLST), SDS-PAGE, and pulsed-field gel electrophoresis (PFGE). The pLVPK-related genetic loci (rmpA2, terW, iutA, and silS) were screened in all CAZ/AVI-resistant CRKP isolates for the presence of virulence plasmids by PCR. Capsule typing, serum killing assay, Galleria mellonella lethality experiments, and mouse lethality assay were conducted to identify CAZ/AVI-resistant hvKP among isolates that carried all four virulence genes.

Results: A total of 232 CRKP isolates were collected. Overall, CAZ/AVI-resistance was found in 8.2% (19/232) CRKP isolates isolated from patients with no history of previous CAZ/AVI-based treatment. Among these, 63.2% (12/19) were metallo-β-lactamase-producing K. pneumoniae (MBL-KP), 52.6% (10/19) were Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP), and 26.3% (5/19) produced both MBL and KPC. The presence of carbapenemase promoted a very high increase in CAZ/AVI minimum inhibitory concentration only when ompk35 and ompk36 were absent. Alarmingly, nine isolates had all four virulence genes for the presence of virulence plasmids. All nine isolates were considered to be CAZ/AVI-resistant hvKP according to the G. mellonella infection model and mouse lethality assay, with ST23 being the most common type (55.6%, 5/9).

Conclusion: The newly emerged hypervirulent CAZ/AVI-resistant KP strain might cause a serious threat to public health, suggesting an urgent need for enhanced clinical awareness and epidemiologic surveillance.

Keywords: Klebsiella pneumoniae; ceftazidime/avibactam-resistant; hypervirulent; ompk35.

Grants and funding

Financial support was provided by the National Natural Science Foundation of China (81860368), the National Mega-project for Innovative Drugs (2019ZX09721001), and Anti-infective drug research (2019BJZDS003).