The clinical relevance of lung ultrasonography (LUS) has been rapidly growing since the 1990s. However, LUS is mainly based on the evaluation of visual artifacts (also called B-lines), leading to subjective and qualitative diagnoses. The formation of B-lines remains unknown and, hence, researchers need to study their origin to allow clinicians to quantitatively evaluate the state of lungs. This paper investigates an ambiguity about the formation of B-lines, leading to the formulation of two main hypotheses. The first hypothesis states that the visualization of these artifacts is linked only to the dimension of the emitted beam, whereas the second associates their appearance to specific resonance phenomena. To verify these hypotheses, the frequency spectrum of B-lines was studied by using dedicated lung-phantoms. A research programmable platform connected to an LA533 linear array probe was exploited both to implement a multifrequency approach and to acquire raw radio frequency data. The strength of each artifact was measured as a function of frequency, focal point, and transmitting aperture by means of the artifact total intensity. The results show that the main parameter that influences the visualization of B-lines is the frequency rather than the focal point or the number of transmitting elements.