Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

N Engl J Med. 2020 Oct 8;383(15):1492-1494. doi: 10.1056/NEJMc2026172. Epub 2020 Sep 16.

Authors

Julia Joung¹, Alim Ladha¹, Makoto Saito², Nam-Gyun Kim³, Ann E Woolley⁴, Michael Segel², Robert P J Barretto⁵, Amardeep Ranu⁶, Rhiannon K Macrae², Guilhem Faure², Eleonora I Ioannidi¹, Rohan N Krajeski¹, Robert Bruneau³, Meei-Li W Huang³, Xu G Yu⁷, Jonathan Z Li⁴, Bruce D Walker⁷, Deborah T Hung², Alexander L Greninger³, Keith R Jerome⁸, Jonathan S Gootenberg⁹, Omar O Abudayyeh⁹, Feng Zhang¹⁰

Affiliations

¹ Massachusetts Institute of Technology, Cambridge, MA.
² Broad Institute of MIT and Harvard, Cambridge, MA.
³ University of Washington, Seattle, WA.
⁴ Brigham and Women's Hospital, Boston, MA.
⁵ Kallyope, New York, NY.
⁶ DynamiCare Health, Boston, MA.
⁷ Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA.
⁸ Fred Hutchinson Cancer Research Center, Seattle, WA.
⁹ Massachusetts Institute of Technology, Cambridge, MA jgoot@mit.edu omarabu@mit.edu.
¹⁰ Broad Institute of MIT and Harvard, Cambridge, MA zhang@broadinstitute.org.

No abstract available

Publication types

Letter
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Betacoronavirus / genetics
Betacoronavirus / isolation & purification*
COVID-19
CRISPR-Cas Systems
Clinical Laboratory Techniques / methods
Clustered Regularly Interspaced Short Palindromic Repeats*
Coronavirus Infections / diagnosis*
Humans
Pandemics
Pneumonia, Viral / diagnosis*
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Sensitivity and Specificity

Substances

RNA, Viral

Grants and funding