We measured severe acute respiratory syndrome coronavirus 2 spike protein subunits S1/S2 antibodies by using capillary electrophoresis and a chemiluminescence immunoassay for 5,444 active healthcare workers in Italy. Seroprevalence was 6.9% and higher among participants having contact with patients. Seroconversion was not observed in 37/213 previously infected participants.
Keywords: COVID-19; Italy; SARS-CoV-2; Turin; age; coronavirus; coronavirus disease; healthcare workers; respiratory infections; risk; screening; seroprevalence; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.