The Perspective of Diagnostic and Prognostic Values of Lipoxygenases mRNA Expression in Colon Adenocarcinoma

Guo-Tian Ruan; Yi-Zhen Gong; Li-Chen Zhu; Feng Gao; Xi-Wen Liao; Xiang-Kun Wang; Guang-Zhi Zhu; Cun Liao; Shuai Wang; Ling Yan; Hai-Lun Xie; Xin Zhou; Jun-Qi Liu; Meng-Nan Shao; Jia-Liang Gan

doi:10.2147/OTT.S251965

The Perspective of Diagnostic and Prognostic Values of Lipoxygenases mRNA Expression in Colon Adenocarcinoma

Onco Targets Ther. 2020 Sep 23:13:9389-9405. doi: 10.2147/OTT.S251965. eCollection 2020.

Authors

Guo-Tian Ruan^#¹, Yi-Zhen Gong^#¹, Li-Chen Zhu², Feng Gao¹, Xi-Wen Liao³, Xiang-Kun Wang³, Guang-Zhi Zhu³, Cun Liao¹, Shuai Wang¹, Ling Yan¹, Hai-Lun Xie¹, Xin Zhou³, Jun-Qi Liu³, Meng-Nan Shao⁴, Jia-Liang Gan¹

Affiliations

¹ Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.
² Department of Immunology, School of Preclinical Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.
³ Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.
⁴ Life Sciences Institute, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.

^# Contributed equally.

Abstract

Background: This study was mainly to explore and study the potential application of lipoxygenases (ALOX) family genes in the diagnostic and prognostic values of colon adenocarcinoma (COAD).

Methods: Data sets related to the ALOX genes of COAD were obtained from The Cancer Genome Atlas and the University of California, Santa Cruz Xena browser. Then, the relevant biological information was downloaded from the public data platform. Finally, the bioinformatics technologies and clinical verification were employed to comprehensively analyze the potential values of ALOX genes.

Results: The Pearson correlation analysis indicated that there were correlations among ALOXE3, ALOX5, ALOX12, and ALOX12B. The diagnostic receiver operating characteristic (ROC) curves suggested that ALOXE3 and ALOX12 had significant diagnosis in COAD: ALOXE3; P<0.001, area under curve (AUC) 95%CI:=0.818 (0.773-0.862) and ALOX12; P<0.001, AUC 95%CI=0.774 (0.682-0.807). Besides, the verification study indicated that ALOX12 had a diagnostic value in COAD. Finally, our multivariate survival analysis and comprehensive prognosis of ALOX genes in COAD suggested that the ALOXE3 and ALOX12 were associated with COAD overall survival: ALOXE3; P=0.025, HR 95%CI=1.765 (1.074-2.901), ALOX12; P=0.046, HR 95%CI=1.680 (1.009-2.796), and the low expression of ALOXE3 and ALOX12 had a favorable prognosis of COAD (all P<0.05); on the contrary, the high regulation of them increased the risk of death.

Conclusion: In our study, we observed that the mRNA expressions of ALOX genes were associated with the diagnosis and prognosis of COAD. The results of the diagnostic analysis suggested that ALOX12 might have a diagnosis value in COAD. Besides, our comprehensive prognosis analysis indicated that ALOXE3 combined ALOX12 might serve as potential prognosis biomarkers for COAD.

Keywords: The Cancer Genome Atlas; colon adenocarcinoma; diagnostic; lipoxygenases; mRNA; prognostic.

Grants and funding

This work was sponsored in part by Innovation Project of Guangxi Graduate Education (JGY2019052) and Self-financing Scientific Research Project of Guangxi Zhuang Autonomous Region Health Commission, China (Z20180959). JGY2019052 and Z20180959 play roles in the analysis, interpretation of data and writing the manuscript.