Objective: To investigate the associations between TNF-α and CCR5Δ32 gene polymorphisms and influenza A(H1N1)pdm09. Methods: Studies in PubMed, Cochrane Library, OVID, EBSCO, Web of Science published before February 7, 2019 were retrieved comprehensively. Observational studies related to TNF-alpha and CCR5 gene polymorphisms and influenza A(H1N1) pdm09 were collected. A strict quality evaluation was carried out according to NOS scale. Meta-analysis was performed using software Revman 5.0 and Stata 11.0. Results: After screening, a total of 8 studies were included in this Meta-analysis. The results showed that TNF-α gene polymorphism rs361525 might be associated with the risk of influenza A(H1N1)pdm09 virus infection (A vs. G: OR=2.25, 95%CI: 1.09-4.65, P=0.03; AA vs. GG: OR=4.34, 95%CI: 1.65-11.41, P=0.003; AA vs. AG+GG: OR=4.38, 95%CI: 1.67-11.48, P=0.003), similar trend also found in rs1800750 (AA+AG vs. GG: OR=2.42, 95%CI: 1.24-4.71, P=0.01). The results of subgroup analysis indicated that A allele and AA+AG genotypes of rs361525 were risk factors for influenza A(H1N1) pdm09 virus infection in Caucasians. AA genotype was a risk factor for influenza A(H1N1) pdm09 virus infection in Mexican (P<0.05). There was no significant difference in the genetic polymorphism of CCR5 and the severity of influenza A (H1N1) pdm09 virus indection (P>0.05). Conclusion: People with allele A or genotype AA at rs361525, genotype AA+AG at rs1800750 of TNF-α gene might be more susceptible to influenza A(H1N1) pdm09.
目的： 探讨肿瘤坏死因子-α（TNF-α）及CCR5Δ32基因多态性与A（H1N1）pdm09的相关性。 方法： 全面检索PubMed、Cochrane Library、OVID、EBSCO、Web of Science于2019年2月7日及以前发表的相关文献，收集TNF-α及CCR5Δ32基因多态性与A（H1N1）pdm09相关的观察性研究，并按照NOS量表进行严格地质量评价，采用Revman 5.0和Stata 11.0软件进行Meta分析。 结果： 经筛选，共8篇符合排除纳入标准的研究被纳入本次Meta分析，结果显示，TNF-α的基因多态性与A（H1N1）pdm09的发病风险可能具有相关性。rs361525位点携带A等位基因的患者A（H1N1）pdm09是G等位基因的2.25倍（A vs. G：OR=2.25，95%CI：1.09~4.65，P=0.03）；rs361525位点AA基因型的携带者感染A（H1N1）pdm09的可能性分别为GG基因型、AG+GG基因型携带者的4.34倍（95%CI：1.65~11.41，P=0.003）、4.38倍（95%CI：1.67~11.48，P=0.003）。rs361525位点的AA+AG基因型可能是人群感染A（H1N1）pdm09的危险因素（rs1800750：AA+AG vs. GG：OR=2.42，95%CI：1.24~4.71，P=0.01）。亚组分析的结果提示，rs361525位点的A等位基因、AA+AG基因型是高加索人种感染A（H1N1）pdm09的危险因素，AA基因型是墨西哥人种感染A（H1N1）pdm09的危险因素（P<0.05）。CCR5的基因多态性与A（H1N1）pdm09的严重程度均无统计学意义（P>0.05）。 结论： TNF-α基因的rs361525位点具有等位基因A或者AA基因型，或rs1800750位点具有基因型AA+AG的人群可能更易感染A（H1N1）pdm09。.
Keywords: CCR; Gene polymorphism; Influenza; TNF-α.