The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review

Nicholas Tripodi; Daniel Corcoran; Peta Antonello; Nicholas Balic; Darcy Caddy; Aidan Knight; Casey Meehan; Fotios Sidiroglou; Sarah Fraser; Dimitrios Kiatos; Maja Husaric; Vasso Apostolopoulos; Jack Feehan

doi:10.1016/j.jphotobiol.2020.112100

The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review

J Photochem Photobiol B. 2021 Jan:214:112100. doi: 10.1016/j.jphotobiol.2020.112100. Epub 2020 Dec 4.

Authors

Affiliations

¹ Institute of Health and Sport, Victoria University, Melbourne, Australia; First Year College, Victoria University, Melbourne, Australia; Osteopathy Group, College of Health and Biomedicine, Victoria University, Melbourne, Australia; Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, Australia. Electronic address: nicholas.tripodi@vu.edu.au.
² Osteopathy Group, College of Health and Biomedicine, Victoria University, Melbourne, Australia.
³ First Year College, Victoria University, Melbourne, Australia; Institute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne, Australia.
⁴ Institute of Health and Sport, Victoria University, Melbourne, Australia.
⁵ Institute of Health and Sport, Victoria University, Melbourne, Australia; Osteopathy Group, College of Health and Biomedicine, Victoria University, Melbourne, Australia.
⁶ Institute of Health and Sport, Victoria University, Melbourne, Australia; First Year College, Victoria University, Melbourne, Australia; Osteopathy Group, College of Health and Biomedicine, Victoria University, Melbourne, Australia.
⁷ Osteopathy Group, College of Health and Biomedicine, Victoria University, Melbourne, Australia; Department of Medicine-Western Health, Melbourne Medical School, The University of Melbourne, St. Albans, Australia; Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, Australia.

PMID: 33316625
DOI: 10.1016/j.jphotobiol.2020.112100

Abstract

Photobiomodulation (PBM) is reported to impart a range of clinical benefits, from the healing of chronic wounds to athletic performance enhancement. The increasing prevalence of this therapy conflicts with the lack of understanding concerning specific cellular mechanisms induced by PBM. Herein, we systematically explore the literature base, specifically related to PBM (within the range 600-1070 nm) and its influence on dermal fibroblasts. The existing research in this field is appraised through five areas: cellular proliferation and viability; cellular migration; ATP production and mitochondrial membrane potential; cellular protein expression and synthesis; and gene expression. This review demonstrates that when fibroblasts are irradiated in vitro within a set range of intensities, they exhibit a multitude of positive effects related to the wound healing process. However, the development of an optimal in vitro framework is paramount to improve the reliability and validity of research in this field.

Keywords: Cellular proliferation; Cellular protein expression; Cellular viability; Gene expression; Low level light therapy; Photobiomodulation.

Publication types

Systematic Review

MeSH terms

Cell Line
Cell Movement / radiation effects
Cell Proliferation / radiation effects
Cell Survival / radiation effects
Fibroblasts / cytology
Fibroblasts / radiation effects*
Gene Expression Regulation / radiation effects
Humans
In Vitro Techniques
Low-Level Light Therapy / methods*
Proteins / genetics
Proteins / metabolism
Skin
Wound Healing / radiation effects*

Substances

Proteins