Brush Samples of Oral Lesions to FTA Elute Card for High-risk Human Papilloma Virus Diagnosis

Anticancer Res. 2021 Jan;41(1):269-277. doi: 10.21873/anticanres.14773.

Abstract

Aim: To investigate the level of agreement between three non-invasive methods for hrHPV diagnosis in oral and oropharyngeal squamous cell carcinoma (OSCC, OPSCC) and in oral mucosal lesions.

Materials and methods: For hrHPV DNA FTA Elute card™ and Anyplex II HPV28™ were used and for hrHPV mRNA PreTect SEE™ in tumour patients (n=60), non-tumour lesions (n=51), immunosuppression or previous hrHPV-infection (n=32).

Results: The level of agreement between the DNA-methods was 82.2% (k=0.54, p=0.001). Pair-wise comparison for the FTA Elute card were close to the reference (AUC=0.83, 95% CI=0.73-0.90). hrHPV mRNA was diagnosed in 50% of the tumours, with an agreement level of 58.3%, compared to Anyplex II (k=0.17, p=0.04). The hrHPV positivity in oral lesions was 3.9% for immunosuppression and for previous HPV infection 9.4%.

Conclusion: The FTA card is reliable for hrHPV DNA diagnosis while mRNA gives an insight into viral activity and correlates with severity of the lesion.

Keywords: DNA; FTA Elute card; hrHPV; human papillomavirus; mRNA; oral- and oropharyngeal cancer; potentially malignant oral disorders; real-time PCR.

MeSH terms

  • Adult
  • Aged
  • Biopsy
  • DNA, Viral
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques
  • Papillomaviridae / genetics*
  • Papillomavirus Infections / complications
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology*
  • Polymerase Chain Reaction
  • Prevalence
  • ROC Curve
  • Squamous Cell Carcinoma of Head and Neck / epidemiology
  • Squamous Cell Carcinoma of Head and Neck / etiology
  • Stomatitis / complications
  • Stomatitis / diagnosis*
  • Stomatitis / virology*
  • Sweden / epidemiology

Substances

  • DNA, Viral