Using BioID to Characterize the RAS Interactome

Methods Mol Biol. 2021:2262:271-280. doi: 10.1007/978-1-0716-1190-6_16.

Abstract

Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.

Keywords: BioID; BirA; HRAS; Interactome; KRAS; NRAS; Proximity labeling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotin / chemistry*
  • Biotinylation / methods*
  • Humans
  • Protein Binding
  • Protein Interaction Domains and Motifs*
  • Protein Interaction Mapping / methods*
  • ras Proteins / chemistry
  • ras Proteins / metabolism*

Substances

  • Biotin
  • ras Proteins