Objective: To investigate the effect of vitamin D3 to platelet activation by tumor cell culture medium.
Methods: The peripheral blood platelets of BALB/c mice were isolated. The platelets were activated in 4T1 culture fluid for 24 h. The platelets were divided into 7 groups: control group, activation group, 1 nmol/L vitamin D3 group, 10 nmol/L vitamin D3 group, 50 nmol/L vitamin D3 group, 100 nmol/L vitamin D3 group, and positive drug (0.1 μmol/L eptifibatide) group. CCK-8 assay was used to detect the platelet proliferation at 24, 48 and 72 h. Flow cytometry was used to detect the expression of CD61 and CD62p and receptor for advanced glycation end products (RAGE) at 24, 48 and 72 h. ELISA was used to detect the level of platelet-endothelial cell adhesion molecule-1 (PECAM-1) at 24, 48 and 72 h.
Results: The CD41+/CD61+ and CD41+/CD62p+ ratio, PECAM-1 content and RAGE expression of platelets in activated group were all significantly increased as compared with those in control group (P<0.05). Compared with the activated group, the platelet proliferation, proportion of CD41+/CD61+ and CD41+/CD62p+, content of PECAM-1 and RAGE expression in vitamin D3 groups were all decreased (P<0.05).
Conclusion: Vitamin D3 shows antiplatelet effect and can inhibit platelet proliferation and activation.
题目: 维生素D3对肿瘤细胞培养液介导血小板活化的影响.
目的: 探讨维生素D3对肿瘤细胞培养液介导血小板活化的影响.
方法: 原代分离BALB/c小鼠外周血血小板，采用小鼠乳腺癌细胞4T1培养液培养血小板24 h进行活化。将血小板分为7组：对照组、活化组、1 nmol/L维生素D3组、10 nmol/L维生素D3组、50 nmol/L维生素D3组、100 nmol/L维生素D3组、阳性药物（0.1 μmol/L依替巴肽）组。CCK-8法检测24、48、72 h血小板增殖能力；流式细胞术检测24、48、72 h血小板活化标志物CD61和CD62p及晚期糖基化终末产物受体（RAGE）的表达水平；ELISA检测24、48、72 h血小板内皮细胞粘附分子1（PECAM-1）的水平.
结果: 与对照组比较，活化组血小板CD41+/CD61+及CD41+/CD62p+比例、PECAM-1含量、RAGE表达均明显升高（P<0.05）。与活化组比较，维生素D3组血小板增殖、CD41+/CD61+及CD41+/CD62p+比例、PECAM-1含量及RAGE表达均明显降低（P<0.05）.
结论: 维生素D3可抑制血小板增殖与活化，具有抗血小板作用.