Mouse Model of Reversible Intestinal Inflammation

Cheong Kc Kwong Chung; Jennifer Brasseit; Esther Althaus-Steiner; Silvia Rihs; Christoph Mueller

doi:10.21769/BioProtoc.2173

Mouse Model of Reversible Intestinal Inflammation

Bio Protoc. 2017 Mar 20;7(6):e2173. doi: 10.21769/BioProtoc.2173.

Authors

Cheong Kc Kwong Chung¹, Jennifer Brasseit¹, Esther Althaus-Steiner¹, Silvia Rihs¹, Christoph Mueller¹

Affiliation

¹ Division of Experimental Pathology, Institute of Pathology, University of Bern, Bern, Switzerland.

Abstract

Current therapies to treat inflammatory bowel disease by dampening excessive inflammatory immune responses have had limited success ( Reinisch et al., 2011 ; Rutgeerts et al., 2005 ; Sandborn et al., 2012 ). To develop new therapeutic interventions, there is a need for better understanding of the mechanisms that are operative during mucosal healing (Pineton de Chambrun et al., 2010 ). To this end, a reversible model of colitis was developed in which colitis induced by adoptive transfer of naïve CD4⁺ CD45RB^hi T cells in lymphopenic mice can be reversed through depletion of colitogenic CD4⁺ T cells ( Brasseit et al., 2016 ).

Keywords: Colitis; Mucosal healing; Relapsing disease; Remission.