Emerging research has highlighted the importance of metabolic pathways and metabolites in dictating immune cell lineage decisions during thymocyte development. Here, we discuss several complementary approaches, including flow cytometry, metabolic flux, and transcriptome analyses, to characterize the dynamic changes in metabolic profiles associated with invariant natural killer T cell development.
Keywords: Bioinformatics; Flow cytometry; Glycolysis; Metabolism; Oxidative phosphorylation; Seahorse; iNKT cell.
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.