Fluorescent In Situ Hybridization (FISH)

Jeanette M Ramos

doi:10.1007/978-1-0716-1948-3_12

Fluorescent In Situ Hybridization (FISH)

Methods Mol Biol. 2022:2422:179-189. doi: 10.1007/978-1-0716-1948-3_12.

Author

Jeanette M Ramos¹

Affiliation

¹ Department of Pathology and Laboratory Services, University of Arkansas for Medical Sciences, Little Rock, AR, USA. JMRamos@uams.edu.

PMID: 34859406
DOI: 10.1007/978-1-0716-1948-3_12

Abstract

In situ hybridization (ISH) is a technique that uses nucleic acid probes to allow for visualization of nucleic acid sequences in cellular/tissue preparations. Both enzymatic and fluorescent ISH studies can be used as adjunct studies, and in some cases are vital to making specific diagnoses. This chapter will focus on fluorescent in situ hybridization.

Keywords: Adjunct study; Chromosome; Deoxyribonucleic acid; Fluorescent; Interphase; Ribonucleic acid.

MeSH terms

DNA Probes
In Situ Hybridization, Fluorescence*
Interphase
Nucleic Acid Hybridization

Substances

DNA Probes