Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation

Teruyoshi Yamashita; Daisuke Kamiya; Nami Kawaraichi; Harufu Toyokawa; Teppei Akaboshi; Makoto Ikeya; Yayoi Toyooka

doi:10.1016/j.scr.2022.102696

Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation

Stem Cell Res. 2022 Apr:60:102696. doi: 10.1016/j.scr.2022.102696. Epub 2022 Feb 1.

Authors

Teruyoshi Yamashita¹, Daisuke Kamiya², Nami Kawaraichi³, Harufu Toyokawa³, Teppei Akaboshi², Makoto Ikeya², Yayoi Toyooka⁴

Affiliations

¹ T-CiRA Discovery, Takeda Pharmaceutical Co., Ltd., Fujisawa, Kanagawa, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.
² Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.
³ Orizuru Therapeutics Inc., Fujisawa, Kanagawa, Japan.
⁴ Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan. Electronic address: yayoi.toyooka@cira.kyoto-u.ac.jp.

PMID: 35144211
DOI: 10.1016/j.scr.2022.102696

Abstract

SOX10 (SRY-box transcription factor 10) is not only a definitive molecular marker of neural crest cells (NCCs) but also an essential transcription factor for the differentiation of NCCs in vertebrate embryogenesis. Here, we report the establishment of a human SOX10 knock-in reporter iPSC line (SOX10-tdT) by CRISPR/Cas9-mediated homologous recombination, in which the expression of SOX10 can be monitored as tdTomato fluorescence. This iPSC line can provide a useful tool to model the differentiation process of human NCCs in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Gene Expression Regulation, Developmental
Humans
Induced Pluripotent Stem Cells* / metabolism
Neural Crest / metabolism
SOXE Transcription Factors / genetics
SOXE Transcription Factors / metabolism

Substances

SOX10 protein, human
SOXE Transcription Factors