Abstract
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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DNA, Recombinant*
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Escherichia coli / genetics*
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Genes*
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Mice / genetics*
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Phenotype
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Plasmids
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Tetrahydrofolate Dehydrogenase / genetics*
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Tetrahydrofolate Dehydrogenase / immunology
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Tetrahydrofolate Dehydrogenase / metabolism
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Trimethoprim / pharmacology
Substances
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DNA, Recombinant
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Trimethoprim
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Tetrahydrofolate Dehydrogenase
Associated data
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GENBANK/M10722
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GENBANK/M10811