Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Nature. 1978 Oct 19;275(5681):617-24. doi: 10.1038/275617a0.

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Recombinant*
  • Escherichia coli / genetics*
  • Genes*
  • Mice / genetics*
  • Phenotype
  • Plasmids
  • Tetrahydrofolate Dehydrogenase / genetics*
  • Tetrahydrofolate Dehydrogenase / immunology
  • Tetrahydrofolate Dehydrogenase / metabolism
  • Trimethoprim / pharmacology

Substances

  • DNA, Recombinant
  • Trimethoprim
  • Tetrahydrofolate Dehydrogenase

Associated data

  • GENBANK/M10722
  • GENBANK/M10811