New antibiotics, chloropolysporins A, B and C, were found in the culture broth of an actinomycete identified as Faenia interjecta sp. nov. They were isolated from the culture filtrate by column chromatography on various resinous adsorbents, followed by preparative reverse phase HPLC. Chloropolysporins A, B and C possessed all the same new aglycone composed of actinoidic acid, 3-chloro-4-hydroxyphenylglycine, N-methyl-p-hydroxyphenylglycine and vancomycinic acid. From elementary analyses and mass spectroscopic measurements, the molecular formulae of chloropolysporins A, B and C appear to be C89H99O39N8Cl3 (MW 2,008), C83H89O34N8Cl3 (MW 1,846) and C77H79O30N8Cl3 (MW 1,700), respectively. Their physico-chemical characterizations including molecular formulae revealed that chloropolysporins A, B and C were new members of glycopeptide antibiotics.