Effects of water restriction and dirt on grooming behavior in neotropical rodents (Trinomys setosus and T. yonenagae) (Echimyidae)

Abstract

Grooming in rodents presents an evolutionarily conserved behavioral pattern that may cause water loss since saliva is deposited during washing onto large body surfaces. Trinomys yonenagae and Trinomys setosus are sister species of spiny rats occurring in Brazil, the former inhabiting a paleodesert of fixed dunes in the Caatinga, the latter being found in mesic environments of the Atlantic Forest. Consequently, it is expected that both species evolved under different selective pressures related to water balance, with T. yonenagae presenting mechanisms for dealing with water deprivation not found in T. setosus. Reduction of self-cleaning expression seems to offer a possible way to save water, as previously suggested by studies of the sand-dwelling spiny rat. Therefore, we propose to investigate grooming under four conditions: 'control' (C), a regimen of 'water restriction' (WR), of 'dirt' (D), and the combination of both conflicting stimuli (WR + D), in T. setosus, T. yonenagae, and Rattus norvegicus to compare the behavioral responses of these species. The main differences are observed in the forest dweller: T. setosus expresses a low relative duration of face washing under C, whose value is intermediate between the ones found in the two other species. WR treatment does not alter this pattern, however, the addition of dirt (D, WR + D) significantly increases the relative duration of washing in relation to C. Locomotor activity is decreased both in T. setosus and Wistar rats when they are under WR, a situation that could jeopardize antipredatory performance. T. yonenagae, the sand dweller, maintains a significantly lower expression of washing under C, as previously suggested, and under WR, D and WR + D. In addition, differently from the other two species the sand dweller maintains a normal activity level during all treatments. This study suggests differences in grooming as a strategy alluding to water balance by the two spiny rats inhabiting different ecosystems. A significantly clear pattern that saves water is observed in T. yonenagae, which probably has contributed to his evolution in one of the hottest semiarid areas of the world.

Keywords: Ecophysiology; Neotropics; Rodentia; Stereotyped behavior; Wistar rat.