Interactions between dissolved organic matter and the microbial community are modified by microplastics and heat waves

Abstract

Dissolved organic matter (DOM) exists widely in natural waters and plays an important role in river carbon cycles and greenhouse gas emissions through microbial interactions. However, information on DOM-microbe associations in response to environmental stress is limited. River environments are the main carriers of microplastic (MP) pollution, and global heat waves (HWs) are threatening river ecology. Here, through MP exposure and HW simulation experiments, we found that DOM molecular weight and aromaticity were closely related to initial microbial communities. Moreover, MP-derived DOM regulated microbial community abundance and diversity, influenced microorganism succession trajectories as deterministic factors, and competed with riverine DOM for microbial utilization. SimulatedHWs enhanced the MP-derived DOM competitive advantage and drove the microbial community to adopt a K-strategy for effective recalcitrant carbon utilization. Relative to single environmental stressor exposure, combined MP pollution and HWs led to a more unstable microbial network. This study addresses how MPs and HWs drive DOM-microbe interactions in rivers, contributes to an in-depth understanding of the fate of river DOM and microbial community succession processes, and narrows the knowledge gap in understanding carbon sinks in aquatic ecosystems influenced by human activities and climate change.

Keywords: Carbon sink; DOM; Heat waves; Microbial community; Microplastics; River.