Green-emitting functionalized silicon nanoparticles as an "off-on" fluorescence bio-probe for the sensitive and selective detection of mercury (II) and 3-mercaptopropionic acid

Abstract

Herein, we developed a class of functionalized silicon nanoparticles (F-SiNPs) bio-probes named thiol-conjugated F-SiNPs. They combine excellent biocompatibility with small dimensions (<10 nm) and biological usefulness with sustained and robust fluorescence (3.32% photoluminescent quantum yield). Identifying 3-Mercaptopropionic acid (3-MPA), which lowers the quantity of gamma-aminobutyric acid in the brain, and mercury (Hg²⁺) was a crucially important step since their excessive levels are a sign of several disorders. Using F-SiNPs as a fluorescent bio-probe, we provided an "off-on" technique for sensitively and selectively determining Hg²⁺ and 3-MPA in this study. The 3-(2-aminoethylamino) propyl (dimethoxymethylsilane) and basic fuchsin as precursors were hydrothermally treated to produce the F-SiNPs exhibiting green fluorescence. Our results suggest that Hg²⁺ reduced the fluorescence of F-SiNPs because of strong ionic interactions and metal-ligand binding among many thiols and carboxyl groupings at the surface of Hg²⁺ and F-SiNPs. Additionally, the resultants demonstrated that after being quenched by Hg²⁺, the produced F-SiNPs led to the distinctive "off-on" response to 3-MPA. Moreover, the method could detect Hg²⁺ and 3-MPA with limits of detection of 0.065 μM and 0.017 μM, respectively. The technique employed is quick, easy, affordable, and environmentally friendly. The sensing platform has successfully determined Hg²⁺ and 3-MPA in urine, water, and human serum samples.

Keywords: 3-Mercaptopropionic acid; Fluorescence; Functionalized-silicon nanoparticles; Human serum; Mercury (II).