Fission yeast obeys a linear size law under nutrient titration

François Bertaux; Istvan T Kleijn; Samuel Marguerat; Vahid Shahrezaei

doi:10.17912/micropub.biology.000833

Fission yeast obeys a linear size law under nutrient titration

MicroPubl Biol. 2023 Apr 28:2023:10.17912/micropub.biology.000833. doi: 10.17912/micropub.biology.000833. eCollection 2023.

Authors

François Bertaux^{1

2

3}, Istvan T Kleijn^{1

2

3}, Samuel Marguerat^{1

2

4}, Vahid Shahrezaei³

Affiliations

¹ MRC London Institute of Medical Sciences, London, England, United Kingdom.
² Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, England, United Kingdom.
³ Department of Mathematics, Faculty of Natural Sciences, Imperial College London, London, England, United Kingdom.
⁴ Current address: UCL Cancer Institute, University College London, London, England, United Kingdom.

Abstract

Steady-state cell size and geometry depend on growth conditions. Here, we use an experimental setup based on continuous culture and single-cell imaging to study how cell volume, length, width and surface-to-volume ratio vary across a range of growth conditions including nitrogen and carbon titration, the choice of nitrogen source, and translation inhibition. Overall, we find cell geometry is not fully determined by growth rate and depends on the specific mode of growth rate modulation. However, under nitrogen and carbon titrations, we observe that the cell volume and the growth rate follow the same linear scaling.

Grants and funding

F Bertaux received financial support from a Leverhulme Research Project Grant (RPG-2014-408) awarded to S Marguerat and V Shahrezaei. IT Kleijn was supported by the Wellcome Trust (108908/Z/15/Z, 203968/Z/16/Z). V Shahrezaei is supported by the EPSRC Centre for Mathematics of Precision Healthcare (EP/N014529/1). S Marguerat was supported by the UK Medical Research Council. This research was funded in whole, or in part, by the Wellcome Trust [108908/Z/15/Z, 203968/Z/16/Z]. For the purpose of open access, the authors have applied a CC-BY public copyright licence to any Author Accepted Manuscript version arising from this submission.