Ranavirus infection does not reduce heat tolerance in a larval amphibian

Abstract

Extreme heat events and emerging infectious diseases negatively impact wildlife populations, but the interacting effects of infection and host heat tolerance remain understudied. The few studies covering this subject have demonstrated that pathogens lower the heat tolerance of their hosts, which places infected hosts at a greater risk experiencing lethal heat stress. Here, we studied how ranavirus infection influenced heat tolerance in larval wood frogs (Lithobates sylvaticus). In line with similar studies, we predicted the elevated costs of ranavirus infection would lower heat tolerance, measured as critical thermal maximum (CT_max), compared to uninfected controls. Ranavirus infection did not reduce CT_max and there was a positive relationship between CT_max and viral loads. Our results demonstrate that ranavirus-infected wood frog larvae had no loss in heat tolerance compared to uninfected larvae, even at viral loads associated with high mortality rates, which contradicts the common pattern for other pathogenic infections in ectotherms. Larval anurans may prioritize maintenance of their CT_max when infected with ranavirus to promote selection of warmer temperatures during behavioral fever that can improve pathogen clearance. Our study represents the first to examine the effect of ranavirus infection on host heat tolerance, and because no decline in CT_max was observed, this suggests that infected hosts would not be under greater risk of heat stress.

Keywords: CT(max); Ranavirus; Thermal tolerance; Viral loads; Wood frogs.