Detailed anatomical study of the peripheral motor branches of the facial nerve in the swine model: A novel investigative approach for facial paralysis research

Abstract

Introduction: Large animal models aid in innovating surgical approaches and developing medical devices for the treatment of facial paralysis. However, there is a lack of information on facial nerve anatomy in swine. This study aimed to investigate the branching pattern and histologic characteristics of the swine facial nerve, thereby establishing the anatomical patterns of preclinical models in facial paralysis research.

Material and methods: The five peripheral motor branches of the facial nerve were dissected in 30 hemifaces of fresh swine cadavers. Starting at the stylomastoid foramen, the course, branching pattern, and diameter of each motor branch was recorded. Samples were taken at the start of each branch for epoxy embedding, toluidine blue staining, and histomorphologic analysis.

Results: The dissections demonstrated consistent anatomy of the buccal and marginal mandibular branches in contrast to the temporal and zygomatic branches, which showed more variation in branching patterns. The buccal branch had the largest mean diameter of 1.34 mm (± 0.26 mm), whereas the marginal mandibular branch had the largest fascicular area of 0.558 mm² (± 0.12 mm²) and highest axon count of 3636.35 (± 526.36). The zygomatic branch had both the smallest diameter of 0.74 mm (± 0.25 mm) and fascicular area of 0.187 mm² (± 0.14 mm²).

Conclusions: The swine facial nerve is anatomically similar to the human facial nerve, making the pig a suitable large animal model. Detailed anatomical and histological information is crucial for developing preclinical models of novel facial nerve reconstruction approaches.

Keywords: Anatomy; Branching pattern; Facial nerve; Histomorphometry; Motor branch; Swine.