Assaying Chemical Long-Term Potentiation in Human iPSC-Derived Neuronal Networks

Methods Mol Biol. 2023:2683:275-289. doi: 10.1007/978-1-0716-3287-1_22.

Abstract

Impairment of long-term potentiation (LTP) is a common feature of many preclinical models of neurological disorders. Modeling LTP on human induced pluripotent stem cells (hiPSC) enables the investigation of this critical plasticity process in disease-specific genetic backgrounds. Here, we describe a method to chemically induce LTP across entire networks of hiPSC-derived neurons on multi-electrode arrays (MEAs) and investigate effects on neuronal network activity and associated molecular changes.

Keywords: Human-induced pluripotent stem cell-derived neurons; Long-term potentiation; Multi-electrode array; Synaptic plasticity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Electrodes
  • Humans
  • Induced Pluripotent Stem Cells*
  • Long-Term Potentiation / physiology
  • Neuronal Plasticity
  • Neurons / physiology