Objective: To investigate the effects of duration, temperature and shake on paraquat (PQ) concentration in the blood of PQ-exposed rats during the specinen preservation and transportation. Methods: In March 2021, 60 SD male rats of Specific Pathogen Free class were randomly divided into low-dose group (10 mg/kg PQ) and high-dose group (80 mg/kg PQ). Each group was divided into 5 subgroups (normal temperature group, cold storage group, 37 ℃ storage group, shaking on normal temperature group and shaking on 37 ℃ group), six rats in each subgroup. The rats were given intraperitoneal injection of PQ, 1 h after exposure, the blood samples were obtained by cardiac extraction. After different interventions, the concentrations of PQ were detected and compared before and after the intervention in each subgroup. Results: In the shaking on 37 ℃ group, the results of PQ concentrations in PQ-exposed rats were significantly lower than those before the intervention (P<0.05). In the other subgroups, the results were not significantly different compared with before intervention (P>0.05) . Conclusion: The concentration of PQ in the blood of rats exposed to PQ was decreased by shaking for 4 hours at 37 ℃.
目的： 探讨在标本保存与运输过程中时长、温度、震荡对百草枯（PQ）染毒大鼠血液中PQ浓度的影响。 方法： 于2021年3月，将无特定病原体（SPF）级SD雄性大鼠60只随机分为低剂量组和高剂量组（PQ染毒剂量分别为10、80 mg/kg），每组30只。每组按干预方法分为常温静置组、冷藏静置组、37 ℃静置组、常温震荡组、37 ℃震荡组5个亚组，每亚组6只大鼠。大鼠通过腹腔注射相应剂量PQ进行染毒，染毒后1 h通过心脏取血的方法获取血液样本，按照不同方式干预后，检测并比较每亚组干预前后的PQ浓度， 结果： 37 ℃震荡组PQ染毒大鼠PQ检测结果较干预前明显下降（P<0.05），其余亚组PQ染毒大鼠PQ检测结果较干预前无明显变化（P>0.05）。 结论： 37 ℃下震荡4 h会使PQ染毒大鼠血液中PQ浓度下降。.
Keywords: Paraquat; Poison detection; Rat; Specimen preservation; Specimen transportation.