Effect of in vitro ribosylation on the dynamic fracture behavior of mature bovine cortical bone

Tanner Snow; William Woolley; Claire Acevedo; Owen T Kingstedt

doi:10.1016/j.jmbbm.2023.106171

Effect of in vitro ribosylation on the dynamic fracture behavior of mature bovine cortical bone

J Mech Behav Biomed Mater. 2023 Dec:148:106171. doi: 10.1016/j.jmbbm.2023.106171. Epub 2023 Oct 17.

Authors

Tanner Snow¹, William Woolley², Claire Acevedo³, Owen T Kingstedt⁴

Affiliations

¹ Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA.
² Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA; Department of Mechanical and Aerospace Engineering, University of California San Diego, San Diego, CA, 92093, USA.
³ Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA; Department of Mechanical and Aerospace Engineering, University of California San Diego, San Diego, CA, 92093, USA. Electronic address: claire.acevedo@utah.edu.
⁴ Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA. Electronic address: o.kingstedt@utah.edu.

PMID: 37890344
DOI: 10.1016/j.jmbbm.2023.106171

Abstract

In this study, the fracture behavior of ribosylated bovine cortical bone is investigated under loading conditions simulating a fall event. Single edge notched specimens, separated into a control group (n = 11) and a ribosylated group (n = 8), were extracted from the mid-diaphysis of a single bovine femur harvested from a mature cow. A seven-day ribosylation process results in the accumulation of Advanced-Glycation End Products (AGEs) cross-links and AGE adducts. Specimens were subjected to symmetric three point bending (opening mode) and an impact velocity of 1.6 m/s using a drop tower. Near-crack displacement fields up to fracture initiation are determined from high-speed images post-processed using digital image correlation. A constrained over-deterministic least squares regression and orthotropic material linear elastic fracture mechanics theory are used to extract the in-plane critical stress intensity factors at fracture initiation (i.e., fracture initiation toughness values). Statistically significant differences were not observed when comparing the in-plane fracture initiation toughness values (p≥0.96) or energy release rate (p=0.90) between the control and seven-day ribosylated groups. The intrinsic variability of bone may require high sample numbers in order to achieve an adequately powered experiment when assessing dynamic fracture behavior. While there are no detectable differences due to the ribosylation treatment investigated, this is likely due to the limited sample sizes utilized.

Keywords: Bovine femur cortical bone; Fracture initiation toughness; Glycation; In vitro ribosylation; Mixed-mode fracture.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Animals
Bone and Bones
Cattle
Cortical Bone
Femur
Fractures, Bone*
Lower Extremity

Abstract

Publication types

MeSH terms

Grants and funding