Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Duan Jinliang; Bai Fang; Zhu Xiaofeng; He Xiaoshun; Hu Anbin

doi:10.1016/j.scr.2023.103302

Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Stem Cell Res. 2024 Mar:75:103302. doi: 10.1016/j.scr.2023.103302. Epub 2024 Jan 5.

Authors

Duan Jinliang¹, Bai Fang¹, Zhu Xiaofeng¹, He Xiaoshun¹, Hu Anbin²

Affiliations

¹ Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China.
² Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China. Electronic address: anbinhu@163.com.

PMID: 38217994
DOI: 10.1016/j.scr.2023.103302

Abstract

CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis, playing an important role in homeostatic and inflammatory responses. A CCL22-overexpressing human induced pluripotent stem cell line (CNNDi001-A-2) was generated by lentiviral transduction to further study the function of CCL22. The cell line was confirmed to have normal proliferation and pluripotency and could be further differentiated into islet cells for cell replacement therapy in diabetes.

MeSH terms

Cell Line
Chemokine CCL22 / genetics
Chemokine CCL22 / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
Islets of Langerhans*
T-Lymphocytes, Regulatory / metabolism

Substances

Chemokine CCL22
CCL22 protein, human