Changes of synaptic vesicles in three-dimensional synapse models by treatment with umbelliferone in scopolamine-induced hippocampal injury model

Ga-Young Choi; Eunyoung Moon; Hyosung Choi; Hee-Seok Kweon

doi:10.1186/s42649-024-00095-y

Changes of synaptic vesicles in three-dimensional synapse models by treatment with umbelliferone in scopolamine-induced hippocampal injury model

Appl Microsc. 2024 Jan 23;54(1):2. doi: 10.1186/s42649-024-00095-y.

Authors

Ga-Young Choi¹, Eunyoung Moon¹, Hyosung Choi¹, Hee-Seok Kweon²

Affiliations

¹ Center of Research Equipment, Korea Basic Science Institute, Cheongju, 28119, Republic of Korea.
² Center of Research Equipment, Korea Basic Science Institute, Cheongju, 28119, Republic of Korea. hskweon@kbsi.re.kr.

Abstract

The neuroprotective effects of umbelliferone (UMB) were visualized in three-dimensional (3D) images on vesicle density changes of organotypic hippocampal slice tissues (OHSCs) induced by scopolamine by high voltage electron microscopy. Observations revealed that the number of vesicles decreased in OHSCs induced by scopolamine, and UMB was found to inhibit scopolamine-induced reduction in vesicles, resulting in an increase in vesicle count. These 3D models provide valuable insight for understanding the increase of synapse vesicles in hippocampal tissues treated with UMB.

Keywords: Electron tomography; High voltage electron microscopy; Hippocampus; Scopolamine; Synaptic vesicle; Umbelliferone.

Grants and funding

2022M3H9A2083956/Ministry of Science and ICT, South Korea