Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT-Orbitrap MS analysis

Raphael Amancio de Jesus; Wenes Ramos da Silva; Alberto Wisniewski Jr; Luís Fernando de Andrade Nascimento; Arie Fitzgerald Blank; Daniel Alves de Souza; Elma Regina Silva de Andrade Wartha; Paulo Cesar de Lima Nogueira; Valéria Regina de Souza Moraes

doi:10.1002/pca.3335

Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT-Orbitrap MS analysis

Phytochem Anal. 2024 Feb 18. doi: 10.1002/pca.3335. Online ahead of print.

Authors

Raphael Amancio de Jesus¹, Wenes Ramos da Silva¹, Alberto Wisniewski Jr¹, Luís Fernando de Andrade Nascimento², Arie Fitzgerald Blank², Daniel Alves de Souza³, Elma Regina Silva de Andrade Wartha³, Paulo Cesar de Lima Nogueira¹, Valéria Regina de Souza Moraes¹

Affiliations

¹ Department of Chemistry, Federal University of Sergipe, São Cristovão, SE, Brazil.
² Department of Agronomic Engineering, Federal University of Sergipe, São Cristovão, SE, Brazil.
³ Department of Nutrition, Federal University of Sergipe, São Cristovão, SE, Brazil.

PMID: 38369344
DOI: 10.1002/pca.3335

Abstract

Introduction: The species Lantana camara is used in folk medicine. The biological activities of this medicinal plant are attributable to the presence of various derivatives of triterpenoids and phenolic compounds present in its preparations, indicating excellent economic potential.

Objective: In this study, the operational conditions of ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) were optimized using Box-Behnken design to improve the total phenolic content (TPC) recovered in hydroethanolic extracts of L. camara leaves.

Material and methods: The TPC, total flavonoid content (TFC), and antioxidant activities of the hydroalcoholic extracts of L. camara, prepared by UAE and MAE under the optimized extraction conditions, were compared with those of the extracts obtained by conventional extraction methods.

Results: Under the optimal conditions, the extracts obtained by UAE (35% ethanol, 25 min, and a solvent-to-solid ratio of 60:1 mL/g) and by MAE (53% ethanol, 15 min, and 300 W) provided high yields of 32.50% and 38.61% and TPC values of 102.89 and 109.83 mg GAE/g DW, respectively. The MAE extract showed the best results with respect to TPC, TFC, and antioxidant activities, followed by extracts obtained by UAE, Soxhlet extraction, decoction, maceration, and infusion, in that order.

Conclusion: The results obtained indicate that L. camara may be used as an important source of antioxidant phenolic compounds to obtain products with high biological and economic potential, especially when the extraction process is performed under appropriate conditions using MAE and/or UAE, employing environmentally friendly solvents such as water and ethanol.

Keywords: Verbenaceae; antioxidant activities; flavonoids; phenylpropanoids.

Abstract

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