A Brain Ischemia-Reperfusion Model for the Study of Tau Phosphorylation and O-GlcNAcylation

Abstract

Cerebral ischemia produces a decrease, loss, or instability of the assembly processes in the neuronal cytoskeleton, related to the alteration in the normal processes of phosphorylation of the Tau protein, triggering its hyperphosphorylation and altering the normal processes of formation of neuronal microtubules. Here we describe the methods used to study the impact of middle cerebral artery occlusion (MCAo) on neurological functions and Tau phosphorylation in Wistar rat brain.

Keywords: Brain ischemia; O-GlcNAcylation; Phosphorylation; Tau protein; Vascular reperfusion.