Protoplast Isolation, Culture, and Regeneration in Common and Tartary Buckwheat

Magdalena Zaranek; Reneé Pérez-Pérez; Joanna Malec; Ewa Grzebelus

doi:10.1007/978-1-0716-3794-4_5

Protoplast Isolation, Culture, and Regeneration in Common and Tartary Buckwheat

Methods Mol Biol. 2024:2791:45-56. doi: 10.1007/978-1-0716-3794-4_5.

Authors

Magdalena Zaranek¹, Reneé Pérez-Pérez¹, Joanna Malec², Ewa Grzebelus³

Affiliations

¹ Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.
² Department of Plant Cytology and Embryology, Faculty of Biology, Jagiellonian University in Kraków, Kraków, Poland.
³ Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Krakow, Poland. ewa.grzebelus@urk.edu.pl.

PMID: 38532091
DOI: 10.1007/978-1-0716-3794-4_5

Abstract

Techniques based on the use of plant protoplasts are a convenient model for better understanding and observing developmental changes in the cells. The establishment of research tools based on protoplasts consists of many steps needed for optimization. Here, we describe the culture of morphogenic callus (MC)- and hypocotyl-derived protoplasts of common (Fagopyrum esculentum Moench) and Tartary (F. tataricum (L.) Gaertn.) buckwheat. Protoplasts embedding in agarose matrix and application of plant hormones, including phytosulfokine (PSK), enable the development of protoplast cultures and plant regeneration.

Keywords: Agarose; Buckwheat; Driselase; Hypocotyls; In vitro cultures; Morphogenic callus; Phytosulfokine; Plant cells.

MeSH terms

Fagopyrum*
Plant Growth Regulators
Protoplasts

Substances

Plant Growth Regulators