Abstract
Gene fusions that bring expression of the lacZ gene under control of transcriptional and trnaslational signals within the transposable element Tn3 have been used to study regulation of Tn3-specified proteins. A gene encoding a 21,355-Mr peptide that represses translocation of Tn3 and acts at the level oquenced; amber, missense, and cis-dominant (operator-constitutive) point mutations in this gene have been isolated and characterized.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics
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Base Sequence
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DNA Transposable Elements*
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Escherichia coli / genetics*
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Genes, Regulator
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Lac Operon
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Molecular Weight
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Mutation
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Plasmids
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Repressor Proteins / physiology*
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Transcription Factors / physiology*
Substances
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Bacterial Proteins
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DNA Transposable Elements
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Repressor Proteins
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Transcription Factors