Abstract
We evaluated the in vitro effects of lyophilization for 2 vesicular stomatitis virus-based vaccines by using 3 stabilizing formulations and demonstrated protective immunity of lyophilized/reconstituted vaccine in guinea pigs. Lyophilization increased stability of the vaccines, but specific vesicular stomatitis virus-based vaccines will each require extensive analysis to optimize stabilizing formulations.
Keywords:
Ebola virus; Lassa virus; animal models; glycoproteins; guinea pigs; lyophilized; vaccines; vesicular stomatitis virus; viruses; zoonoses.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, N.I.H., Extramural
MeSH terms
-
Animals
-
Antibodies, Viral / blood
-
Antibodies, Viral / immunology
-
Disease Models, Animal*
-
Freeze Drying*
-
Guinea Pigs
-
Vaccine Efficacy
-
Vesicular Stomatitis* / immunology
-
Vesicular Stomatitis* / prevention & control
-
Vesicular Stomatitis* / virology
-
Vesicular stomatitis Indiana virus / immunology
-
Vesiculovirus / genetics
-
Vesiculovirus / immunology
-
Viral Vaccines* / administration & dosage
-
Viral Vaccines* / immunology
Substances
-
Viral Vaccines
-
Antibodies, Viral