Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk

Yu-Ting Wang; Yu-Chun Lin; Yi-Huei Hsieh; Yu-Tzu Lin; Moriyuki Hamada; Chih-Chieh Chen; Jong-Shian Liou; Ai-Yun Lee; Wei-Ling Zhang; Yung-Tsung Chen; Chien-Hsun Huang

doi:10.3390/pathogens13040343

Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk

Pathogens. 2024 Apr 21;13(4):343. doi: 10.3390/pathogens13040343.

Authors

Yu-Ting Wang¹, Yu-Chun Lin^{2

3}, Yi-Huei Hsieh², Yu-Tzu Lin⁴, Moriyuki Hamada⁵, Chih-Chieh Chen^{6

7}, Jong-Shian Liou⁸, Ai-Yun Lee⁸, Wei-Ling Zhang⁸, Yung-Tsung Chen⁹, Chien-Hsun Huang⁸

Affiliations

¹ Division of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, Taipei 115021, Taiwan.
² Taiwan Livestock Research Institute, Ministry of Agriculture, Tainan 71246, Taiwan.
³ Fisheries Research Institute, Ministry of Agriculture, Keelung 202008, Taiwan.
⁴ Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404328, Taiwan.
⁵ Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu 292-0818, Chiba, Japan.
⁶ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
⁷ Rapid Screening Research Center for Toxicology and Biomedicine, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
⁸ Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, Hsinchu 30062, Taiwan.
⁹ Department of Food Science, National Taiwan Ocean University, Keelung 202301, Taiwan.

Abstract

A novel coagulase-negative Staphylococcus strain (H164^T) was isolated from soymilk in Taiwan. Comparative sequence analysis of the 16S rRNA gene revealed that the H164^T strain is a member of the genus Staphylococcus. We used multilocus sequence analysis (MLSA) and phylogenomic analyses to demonstrate that the novel strain was closely related to Staphylococcus gallinarum, Staphylococcus nepalensis, Staphylococcus cohnii, and Staphylococcus urealyuticus. The average nucleotide identity and digital DNA-DNA hybridization values between H164^T and its closest relatives were <95% and <70%, respectively. The H164^T strain could also be distinguished from its closest relatives by the fermentation of _d-fructose, _d-maltose, _d-trehalose, and _d-mannitol, as well as by the activities of α-glucosidase and alkaline phosphatase. The major cellular fatty acids were C15:0 iso and C15:0 anteiso, and the predominant menaquinones were MK-7 and MK-8, respectively. The major cellular fatty acids and predominant menaquinones were C_15:0 iso and C_15:0 anteiso and MK-7 and MK-8, respectively. In conclusion, this strain represents a novel species, named Staphylococcus hsinchuensis sp. nov., with the type strain H164^T (=BCRC 81404^T = NBRC 116174^T).

Keywords: Staphylococcus hsinchuensis; new taxa; polyphasic analysis; soymilk.

Abstract

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