Enzyme modified biodegradable plastic preparation and performance in anaerobic co-digestion with food waste

Wenjie Liu; Shizhuo Wang; Songting He; Yang Shi; Cheng Hou; Xintong Jiang; Yuanbo Song; Tao Zhang; Yalei Zhang; Zheng Shen

doi:10.1016/j.biortech.2024.130739

Enzyme modified biodegradable plastic preparation and performance in anaerobic co-digestion with food waste

Bioresour Technol. 2024 Jun:401:130739. doi: 10.1016/j.biortech.2024.130739. Epub 2024 Apr 25.

Authors

Wenjie Liu¹, Shizhuo Wang², Songting He¹, Yang Shi¹, Cheng Hou², Xintong Jiang¹, Yuanbo Song¹, Tao Zhang², Yalei Zhang³, Zheng Shen⁴

Affiliations

¹ Institute of New Rural Development, School of Electronics and Information Engineering, Tongji University, Shanghai, 201804, P. R. China.
² State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China; Shanghai Research Institute of Pollution Control and Ecological Safety, Tongji University, Shanghai 200092, P. R. China.
³ Institute of New Rural Development, School of Electronics and Information Engineering, Tongji University, Shanghai, 201804, P. R. China; State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China; Key Laboratory of Rural Toilet and SewageTreatment Technology, Ministry of Agricultureand Rural Affairs, Tongji University, Shanghai 201804, P. R. China; Shanghai Research Institute of Pollution Control and Ecological Safety, Tongji University, Shanghai 200092, P. R. China.
⁴ Institute of New Rural Development, School of Electronics and Information Engineering, Tongji University, Shanghai, 201804, P. R. China; State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China; Key Laboratory of Rural Toilet and SewageTreatment Technology, Ministry of Agricultureand Rural Affairs, Tongji University, Shanghai 201804, P. R. China; Shanghai Research Institute of Pollution Control and Ecological Safety, Tongji University, Shanghai 200092, P. R. China. Electronic address: shenzheng@tongji.edu.cn.

PMID: 38670291
DOI: 10.1016/j.biortech.2024.130739

Abstract

A modified biodegradable plastic (PLA/PBAT) was developed by through covalent bonding with proteinase K, porcine pancreatic lipase, or amylase, and was then investigated in anaerobic co-digestion mixed with food waste. Fluorescence microscope validated that enzymes could remain stable in modified the plastic, even after co-digestion. The results of thermophilic anaerobic co-digestion showed that, degradation of the plastic modified with Proteinase K increased from 5.21 ± 0.63 % to 29.70 ± 1.86 % within 30 days compare to blank. Additionally, it was observed that the cumulative methane production increased from 240.9 ± 0.5 to 265.4 ± 1.8 mL/gVS, and the methane production cycle was shortened from 24 to 20 days. Interestingly, the kinetic model suggested that the modified the plastic promoted the overall hydrolysis progression of anaerobic co-digestion, possibly as a result of the enhanced activities of Bacteroidota and Thermotogota. In conclusion, under anaerobic co-digestion, the modified the plastic not only achieved effective degradation but also facilitated the co-digestion process.

Keywords: Anaerobic digestion; Enzyme modified biodegradable plastic; Food waste; Microbial analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amylases / metabolism
Anaerobiosis
Animals
Biodegradable Plastics* / chemistry
Biodegradation, Environmental
Food
Food Loss and Waste
Hydrolysis
Kinetics
Lipase / metabolism
Methane* / metabolism
Refuse Disposal / methods
Swine
Waste Products

Substances

Methane
Biodegradable Plastics
Lipase
Waste Products
Amylases
Food Loss and Waste