O-GlcNAcylation of RBM14 contributes to elevated cellular O-GlcNAc through regulation of OGA protein stability

Tae Hyun Kweon; Hyeryeon Jung; Jeong Yeon Ko; Jingu Kang; Wonyoung Kim; Yeolhoe Kim; Han Byeol Kim; Eugene C Yi; Nam-On Ku; Jin Won Cho; Won Ho Yang

doi:10.1016/j.celrep.2024.114163

O-GlcNAcylation of RBM14 contributes to elevated cellular O-GlcNAc through regulation of OGA protein stability

Cell Rep. 2024 Apr 27;43(5):114163. doi: 10.1016/j.celrep.2024.114163. Online ahead of print.

Authors

Tae Hyun Kweon¹, Hyeryeon Jung², Jeong Yeon Ko³, Jingu Kang¹, Wonyoung Kim³, Yeolhoe Kim³, Han Byeol Kim⁴, Eugene C Yi², Nam-On Ku⁵, Jin Won Cho⁶, Won Ho Yang⁷

Affiliations

¹ Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, Seoul 03722, Republic of Korea; Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
² Department of Molecular Medicine and Biopharmaceutical Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Seoul 03080, Republic of Korea.
³ Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
⁴ Mitohormesis Research Center, Yonsei University Wonju College of Medicine, Wonju 26426, Republic of Korea.
⁵ Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
⁶ Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, Seoul 03722, Republic of Korea; Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea. Electronic address: chojw311@yonsei.ac.kr.
⁷ Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea. Electronic address: bionicwono@yonsei.ac.kr.

PMID: 38678556
DOI: 10.1016/j.celrep.2024.114163

Abstract

Dysregulation of O-GlcNAcylation has emerged as a potential biomarker for several diseases, particularly cancer. The role of OGT (O-GlcNAc transferase) in maintaining O-GlcNAc homeostasis has been extensively studied; nevertheless, the regulation of OGA (O-GlcNAcase) in cancer remains elusive. Here, we demonstrated that the multifunctional protein RBM14 is a regulator of cellular O-GlcNAcylation. By investigating the correlation between elevated O-GlcNAcylation and increased RBM14 expression in lung cancer cells, we discovered that RBM14 promotes ubiquitin-dependent proteasomal degradation of OGA, ultimately mediating cellular O-GlcNAcylation levels. In addition, RBM14 itself is O-GlcNAcylated at serine 521, regulating its interaction with the E3 ligase TRIM33, consequently affecting OGA protein stability. Moreover, we demonstrated that mutation of serine 521 to alanine abrogated the oncogenic properties of RBM14. Collectively, our findings reveal a previously unknown mechanism for the regulation of OGA and suggest a potential therapeutic target for the treatment of cancers with dysregulated O-GlcNAcylation.

Keywords: CP: Molecular biology; Cancer; O-GlcNAc; O-GlcNAcylation; OGA; OGT; Post translational modification; RBM14; TRIM33.