Synergistic and antibiofilm activity of DNase I and glucose oxidase loaded chitosan nanoparticles against dual-species biofilms of Listeria monocytogenes and Salmonella

Quan Lin; Maokun Sheng; Zhaodi Kang; Jiaman Xu; Yan Gao; Su Ma; Bingchang Xin; Yulong Tan

doi:10.1016/j.ijbiomac.2024.131943

Synergistic and antibiofilm activity of DNase I and glucose oxidase loaded chitosan nanoparticles against dual-species biofilms of Listeria monocytogenes and Salmonella

Int J Biol Macromol. 2024 Apr 28;269(Pt 2):131943. doi: 10.1016/j.ijbiomac.2024.131943. Online ahead of print.

Authors

Quan Lin¹, Maokun Sheng¹, Zhaodi Kang¹, Jiaman Xu¹, Yan Gao², Su Ma³, Bingchang Xin⁴, Yulong Tan⁵

Affiliations

¹ College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, China; Shandong Technology Innovation Center of Special Food, Qingdao, China; Qingdao Special Food Research Institute, Qingdao, China.
² Marine Science Research Institute of Shandong Province (National Oceanographic Center of Qingdao), Qingdao, China.
³ State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
⁴ Department of Cariology and Endodontology, Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, China.
⁵ College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, China; Shandong Technology Innovation Center of Special Food, Qingdao, China; Qingdao Special Food Research Institute, Qingdao, China. Electronic address: tanyulong@qau.edu.cn.

PMID: 38688332
DOI: 10.1016/j.ijbiomac.2024.131943

Abstract

Salmonella and Listeria monocytogenes are two of the most common foodborne pathogens in the food industry. They form dual-species biofilms, which have a higher sensitivity to antimicrobial treatment and a greater microbial adhesion. In this experiment, we loaded DNase I and glucose oxidase (GOX) on chitosan nanoparticles (CSNPs) to explore their inhibitory effects on and disruption of dual-species biofilms of Salmonella enterica and L. monocytogenes. Transmission electron microscopy (TEM) showed that CSNP-DNase-GOX and CSNPs were spherical in shape. CSNP-DNase-GOX was shifted and altered compared to the infrared peaks of CSNPs. CSNPs loaded with DNase I and GOX showed an increase in the particle size and an alteration in the polydispersity index (PDI) and the zeta potential. Compared to free DNase I or GOX, DNase I and GOX loaded on CSNPs had higher stability at different temperatures. CSNP-DNase-GOX was more effective in inhibiting dual-species biofilms than CSNP-GOX. Scanning electron microscopy (SEM) and fluorescence microscopy were used to observe the structure of the biofilm, which further illustrated that CSNP-DNase-GOX disrupted the dual-species biofilms of S. enterica and L. monocytogenes.

Keywords: Antibacterial; Enzyme; Extracellular polymers.