Dietary Bacillus velezensis T23 fermented products supplementation improves growth, hepatopancreas and intestine health of Litopenaeus vannamei

Hongwei Yang; Dongdong Du; Qingshuang Zhang; Tsegay Teame; Anran Wang; Qiang Hao; Shubin Liu; Qianwen Ding; Yuanyuan Yao; Yalin Yang; Chao Ran; Shengkang Li; Zhen Zhang; Zhigang Zhou

doi:10.1016/j.fsi.2024.109595

Dietary Bacillus velezensis T23 fermented products supplementation improves growth, hepatopancreas and intestine health of Litopenaeus vannamei

Fish Shellfish Immunol. 2024 Apr 30:149:109595. doi: 10.1016/j.fsi.2024.109595. Online ahead of print.

Authors

Hongwei Yang¹, Dongdong Du², Qingshuang Zhang², Tsegay Teame³, Anran Wang², Qiang Hao⁴, Shubin Liu⁵, Qianwen Ding², Yuanyuan Yao⁵, Yalin Yang⁵, Chao Ran⁵, Shengkang Li⁶, Zhen Zhang⁷, Zhigang Zhou⁸

Affiliations

¹ China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Institute of Marine Sciences, Shantou University, Shantou, 515063, China.
² China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
³ China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Tigray Agricultural Research Institute (TARI), Mekelle, Tigray, Ethiopia.
⁴ China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Norway-China Joint Lab on Fish Gut Microbiota, Department of Biology, Norwegian University of Science and Technology, Trondheim, 7491, Norway.
⁵ Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
⁶ Institute of Marine Sciences, Shantou University, Shantou, 515063, China.
⁷ Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Faculty of Land and Food Systems, The University of British Columbia, Vancouver, Canada. Electronic address: zhangzhen@caas.cn.
⁸ China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Kunpeng Institute of Modern Agriculture of Foshan, Chinese Academy of Agricultural Sciences, Foshan, 528225, China. Electronic address: zhouzhigang03@caas.cn.

PMID: 38692381
DOI: 10.1016/j.fsi.2024.109595

Abstract

This study aimed to elucidate the effects of dietary fermented products of Bacillus velezensis T23 on the growth, immune response and gut microbiota in Pacific white shrimp (Litopenaeus vannamei). Shrimp were fed with diets containing fermentation products of B. velezensis T23 at levels of (0, 0.05, 0.1, 0.2, 0.3, 0.4, and 0.5 g/kg) for 4 weeks, to assess the influence on shrimp growth. The results showed that 0.3 and 0.4 g/kg T23 supplementation improved shrimp growth and feed utilization. Based on these results we selected these three diets (Control, 0.3T23 and 0.4T23) to assess the effect on immune response and gut microbiota of shrimp. Compared with the control, the 0.3T23 and 0.4T23 groups enhanced lipase and α-amylase activities in the gut significantly. Moreover, the 0.4T23 group decreased TAG and MDA levels in hepatopancreas, ALT and AST levels of serum significantly (P < 0.05). In hepatopancreas, CAT and SOD activities were improved observably and the MDA content was reduced markedly in both T23 groups. The expressions of antimicrobial related genes, Cru and peroxinectin in the 0.3T23 group, and proPO and peroxinectin in the 0.4T23 group were up-regulated remarkably (P < 0.05). Moreover, hepatopancreas of shrimp fed with a diet amended with T23 showed a significant down-regulated expression of nf-kb and tnf-α genes, while expressions of tgf-β was considerably up-regulated. Furthermore, serum LPS and LBP contents were reduced markedly in T23 groups. Intestinal SOD and CAT were noteworthy higher in T23 groups (P < 0.05). In the intestine of shrimp fed on the diet enriched with T23 the expression of nf-κb and tnf-α exhibited markedly down-regulated, whereas hif1α was up-regulated (P < 0.05). Besides, in the intestine of shrimp grouped under T23, Cru and peroxinectin genes were markedly up-regulated (P < 0.05). Dietary 0.3 g/kg T23 also upregulated the ratio of Rhodobacteraceae to Vibrionaceae in the gut of the shrimp. Taken together, the inclusion of B. velezensis T23 in the diet of shrimp enhanced the growth and feed utilization, enhanced hepatopancreas and intestine health.

Keywords: Bacillus velezensis T23; Fermented products; Growth; Gut microbiota; Immunity; Shrimp.