Comparative characterisation of extracellular vesicles from canine and human plasma: a necessary step in biomarker discovery

Stephanie Marie Bollard; J Howard; C Casalou; L Mooney; S Peters; C Sweeney; A Ajaykumar; K Triana; A McCann; P A Kelly; S M Potter

doi:10.1007/s11259-024-10405-0

Comparative characterisation of extracellular vesicles from canine and human plasma: a necessary step in biomarker discovery

Vet Res Commun. 2024 May 8. doi: 10.1007/s11259-024-10405-0. Online ahead of print.

Authors

Stephanie Marie Bollard^{1

2

3}, J Howard^{4

5}, C Casalou⁶, L Mooney⁷, S Peters⁷, C Sweeney⁷, A Ajaykumar⁸, K Triana⁸, A McCann^{4

5}, P A Kelly⁷, S M Potter^{4

9}

Affiliations

¹ UCD School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland. stephanie.bollard1@ucdconnect.ie.
² UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland. stephanie.bollard1@ucdconnect.ie.
³ Department of Plastic & Reconstructive Surgery, Mater Misercordiae University Hospital, Eccles Street, Dublin 7, Ireland. stephanie.bollard1@ucdconnect.ie.
⁴ UCD School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
⁵ UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
⁶ UCD Charles Institute of Dermatology, University College Dublin, Belfield, Dublin 4, Ireland.
⁷ UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
⁸ UCD Clinical Research Centre, University College Dublin, Belfield, Dublin 4, Ireland.
⁹ Department of Plastic & Reconstructive Surgery, Mater Misercordiae University Hospital, Eccles Street, Dublin 7, Ireland.

PMID: 38717732
DOI: 10.1007/s11259-024-10405-0

Abstract

Extracellular Vesicles (EV) have become an interesting focus as novel biomarkers of disease and are increasingly reported upon in humans and other species. The Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018) guidelines were published to improve rigor and standardisation within the EV field and provide a framework for the reliable isolation and characterisation of EV populations. However, this rigor and standardisation has been challenging in the area of comparative medicine. Herein we present the successful isolation of EVs from human and canine plasma using Size Exclusion Chromatography and characterise these EVs according to best international practice. This study provides evidence for the reliable comparison of human and canine EVs isolated by this approach, and a baseline description of the EVs from healthy dogs to inform future biomarker studies. This work also demonstrates that the MISEV2018 guidelines can be successfully applied to EVs isolated from canine plasma.

Keywords: Canine; Comparative medicine; Extracellular vesicles; OneHealth; Size exclusion chromatography.