Detection of alkaline phosphatase activity with a CsPbBr3/Y6 heterojunction-based photoelectrochemical sensor

Rong Shao; Lei Deng; Shujun Hu; Minghui Yang; Anjie Min

doi:10.1007/s00604-024-06393-2

Detection of alkaline phosphatase activity with a CsPbBr₃/Y6 heterojunction-based photoelectrochemical sensor

Mikrochim Acta. 2024 May 10;191(6):316. doi: 10.1007/s00604-024-06393-2.

Authors

Rong Shao¹, Lei Deng¹, Shujun Hu^{2

3}, Minghui Yang⁴, Anjie Min^{5

6}

Affiliations

¹ Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
² Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, 410083, China.
³ Research Center of Oral and Maxillofacail Tumor, Xiangya Hospital, Central South University, Changsha, 410083, China.
⁴ Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China. yangminghui@csu.edu.cn.
⁵ Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, 410083, China. william0732@csu.edu.cn.
⁶ Research Center of Oral and Maxillofacail Tumor, Xiangya Hospital, Central South University, Changsha, 410083, China. william0732@csu.edu.cn.

PMID: 38724679
DOI: 10.1007/s00604-024-06393-2

Abstract

An ultra-sensitive photoelectrochemical (PEC) sensor based on perovskite composite was developed for the determination of alkaline phosphatase (ALP) in human serum. In contrast to CsPbBr₃ or Y6 that generated anodic current, the heterojunction of CsPbBr₃/Y6 promoted photocarriers to separate and generated cathodic photocurrent. Ascorbic acid (AA) was produced by ALP hydrolyzing L-ascorbic acid 2-phosphate trisodium salt (AAP), which can combine with the holes on the photoelectrode surface, accelerating the transmission of photogenerated carriers, leading to enhanced photocurrent intensity. Thus, the enhancement of PEC current was linked to ALP activity. The PEC sensor exhibits good sensitivity for detection of ALP owing to the unique photoelectric properties of the CsPbBr₃/Y6 heterojunction. The detection limit of the sensor was 0.012 U·L^-1 with a linear dynamic range of 0.02-2000 U·L^-1. Therefore, this PEC sensing platform shows great potential for the development of different PEC sensors.

Keywords: Heterojunction alkaline phosphatase; Organic–inorganic; Perovskite; Photoelectrochemical.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase* / blood
Alkaline Phosphatase* / chemistry
Alkaline Phosphatase* / metabolism
Ascorbic Acid* / analogs & derivatives
Ascorbic Acid* / blood
Ascorbic Acid* / chemistry
Biosensing Techniques / methods
Calcium Compounds / chemistry
Electrochemical Techniques* / instrumentation
Electrochemical Techniques* / methods
Electrodes*
Humans
Limit of Detection*
Oxides* / chemistry
Photochemical Processes*
Titanium* / chemistry

Substances

Alkaline Phosphatase
Ascorbic Acid
Titanium
Oxides
Calcium Compounds
perovskite
ascorbate-2-phosphate