Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies

Piao Zhao; Lin Feng; Weidan Jiang; Pei Wu; Yang Liu; Hongmei Ren; Xiaowan Jin; Lu Zhang; Haifeng Mi; Xiaoqiu Zhou

doi:10.1186/s40104-024-01023-6

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies

J Anim Sci Biotechnol. 2024 May 12;15(1):72. doi: 10.1186/s40104-024-01023-6.

Authors

Piao Zhao¹, Lin Feng^{1

2

3}, Weidan Jiang^{1

2

3}, Pei Wu^{1

2

3}, Yang Liu^{1

2

3}, Hongmei Ren^{1

2

3}, Xiaowan Jin^{1

2

3}, Lu Zhang⁴, Haifeng Mi⁴, Xiaoqiu Zhou^{5

6

7}

Affiliations

¹ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
² Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
³ Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Chengdu, 611130, Sichuan, China.
⁴ Tongwei Co., Ltd., Healthy Aquaculture Key Laboratory of Sichuan Province, Chengdu, 610041, Sichuan, China.
⁵ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. zhouxq@sicau.edu.cn.
⁶ Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. zhouxq@sicau.edu.cn.
⁷ Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Chengdu, 611130, Sichuan, China. zhouxq@sicau.edu.cn.

Abstract

Background: Ochratoxin A (OTA), a globally abundant and extremely hazardous pollutant, is a significant source of contamination in aquafeeds and is responsible for severe food pollution. The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear. This study screened the substance curcumin (Cur), which had the best effect in alleviating OTA inhibition of myoblast proliferation, from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro.

Methods: A total of 720 healthy juvenile grass carp, with an initial average body weight of 11.06 ± 0.05 g, were randomly assigned into 4 groups: the control group (without OTA and Cur), 1.2 mg/kg OTA group, 400 mg/kg Cur group, and 1.2 mg/kg OTA + 400 mg/kg Cur group. Each treatment consisted of 3 replicates (180 fish) for 60 d.

Results: Firstly, we cultured, purified, and identified myoblasts using the tissue block culture method. Through preliminary screening and re-screening of 96 substances, we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect. Secondly, Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins (MyoG and MYHC) in vivo and in vitro and improve the growth performance of grass carp. Then, Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins (S6K1 and TOR), which was related to the activation of the AKT/TOR signaling pathway. Finally, Cur could downregulate the expression of OTA-enhanced protein degradation-related genes (murf1, foxo3a, and ub), which was related to the inhibition of the FoxO3a signaling pathway.

Conclusions: In summary, our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro. This study confirms the rapidity, feasibility, and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

Keywords: Curcumin; Grass carp; Myotoxic; Ochratoxin A; Protein deposition.

Abstract

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