Mineralization, degradation and osteogenic property of polylactide multicomponent porous composites for bone repair: In vitro and in vivo study

Shanyun Sun; Bin Liang; Zhaowei Yin; Shaowei Pan; Chen Shi; Chao Guo; Zhihai Huang; Chenglin Chu; Yinsheng Dong

doi:10.1016/j.ijbiomac.2024.132378

Mineralization, degradation and osteogenic property of polylactide multicomponent porous composites for bone repair: In vitro and in vivo study

Int J Biol Macromol. 2024 May 23;271(Pt 1):132378. doi: 10.1016/j.ijbiomac.2024.132378. Online ahead of print.

Authors

Shanyun Sun¹, Bin Liang², Zhaowei Yin², Shaowei Pan², Chen Shi², Chao Guo¹, Zhihai Huang¹, Chenglin Chu¹, Yinsheng Dong³

Affiliations

¹ School of Materials Science and Engineering, Southeast University, Nanjing 211189, China; Jiangsu Key Laboratory of Advanced Metallic Materials, Nanjing 211189, China.
² Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
³ School of Materials Science and Engineering, Southeast University, Nanjing 211189, China; Jiangsu Key Laboratory of Advanced Metallic Materials, Nanjing 211189, China. Electronic address: dongys@seu.edu.cn.

PMID: 38750853
DOI: 10.1016/j.ijbiomac.2024.132378

Abstract

Gelatin and hydroxyapatite were assembled into polylactide porous matrix to prepare multicomponent porous composites for bone repair (PLA-gH). PLA-gH possessed a superior ability of mineralization. During simulated body fluids (SBF), the spherical Ca-P depositions on surface of PLA-gH became bulk as Ca/P decreased, while they locally turned into the rod with different variation in Ca/P during SBF containing bovine serum albumin (SBF-BSA), indicating that the mineralization of PLA-gH could be regulated by BSA. Meanwhile, PLA-gH possessed good degradation behaviour, especially in SBF-BSA, the degradation of PLA porous matrix was higher than that in SBF after 14-day immersion, whose crystallinity (X_c) decreased to a slightly lower level. Gelatin and hydroxyapatite endowed PLA-gH with good osteogenic property, characterized by obvious osteogenic differentiation and bone regeneration. In terms of predicting the cytocompatibility, osteogenic differentiation and new bone mineralization of PLA-gH by in vitro methods, applying SBF-BSA may be more reliable than SBF.

Keywords: BSA; Osteogenesis; PLA porous composites.