Differential Resistance to Acetyl-CoA Carboxylase Inhibitors in Rice: Insights from Two Distinct Target-Site Mutations

Zhenzhen Zhou; Qun Jiang; Zeyu Qiu; Xiaodong Hou; Xia Yang; Yuwen Yang; Tingting Hao; Dongshu Guo; Jinyan Wang; Yongfeng Li; Qing Liu; Xitie Ling; Baolong Zhang

doi:10.1021/acs.jafc.4c01889

Differential Resistance to Acetyl-CoA Carboxylase Inhibitors in Rice: Insights from Two Distinct Target-Site Mutations

J Agric Food Chem. 2024 May 29;72(21):12029-12044. doi: 10.1021/acs.jafc.4c01889. Epub 2024 May 16.

Authors

Zhenzhen Zhou^{1

2}, Qun Jiang^{1

3}, Zeyu Qiu^{1

2}, Xiaodong Hou^{1

2}, Xia Yang^{1

2}, Yuwen Yang^{1

2}, Tingting Hao^{1

2}, Dongshu Guo^{1

2}, Jinyan Wang^{1

2}, Yongfeng Li^{1

2}, Qing Liu^{1

2

4}, Xitie Ling^{1

2}, Baolong Zhang^{1

2

3}

Affiliations

¹ Provincial Key Laboratory of Agrobiology and Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing Jiangsu 210014, China.
² Zhongshan Biological Breeding Laboratory, Nanjing Jiangsu 210014, China.
³ Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya Hainan 572025, China.
⁴ College of Agriculture, Yangzhou University, Yangzhou Jiangsu 225009, China.

PMID: 38752706
DOI: 10.1021/acs.jafc.4c01889

Abstract

Weeds present a significant challenge to agricultural productivity, and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides have proven to be effective in managing weed populations in rice fields. To develop ACCase-inhibiting herbicide-resistant rice, we generated mutants of rice ACCase (OsACC) featuring Ile-1792-Leu or Gly-2107-Ser substitutions through ethyl methyl sulfonate (EMS) mutagenesis. The Ile-1792-Leu mutant displayed cross-resistance to aryloxyphenoxypropionate (APP) and phenylpyrazoline (DEN) herbicides, whereas the Gly-2107-Ser mutants primarily exhibited cross-resistance to APP herbicides with diminished resistance to the DEN herbicide. In vitro assays of the OsACC activity revealed an increase in resistance to haloxyfop and quizalofop, ranging from 4.84- to 29-fold in the mutants compared to that in wild-type. Structural modeling revealed that both mutations likely reduce the binding affinity between OsACC and ACCase inhibitors, thereby imparting resistance. This study offers insights into two target-site mutations, contributing to the breeding of herbicide-resistant rice and presenting alternative weed management strategies in rice cultivation.

Keywords: ACCase-inhibiting herbicide; EMS mutagenesis; acetyl-CoA carboxylase; herbicide resistance; rice.

MeSH terms

Acetyl-CoA Carboxylase* / antagonists & inhibitors
Acetyl-CoA Carboxylase* / chemistry
Acetyl-CoA Carboxylase* / genetics
Acetyl-CoA Carboxylase* / metabolism
Enzyme Inhibitors* / chemistry
Enzyme Inhibitors* / pharmacology
Herbicide Resistance* / genetics
Herbicides* / chemistry
Herbicides* / pharmacology
Mutation*
Oryza* / enzymology
Oryza* / genetics
Plant Proteins* / antagonists & inhibitors
Plant Proteins* / chemistry
Plant Proteins* / genetics
Plant Proteins* / metabolism
Plant Weeds / drug effects
Plant Weeds / enzymology
Plant Weeds / genetics

Substances

Acetyl-CoA Carboxylase
Herbicides
Plant Proteins
Enzyme Inhibitors