Monoclonal antibody reactive with the human epidermal-growth-factor receptor recognizes the blood-group-A antigen

Biosci Rep. 1983 Nov;3(11):1045-52. doi: 10.1007/BF01121031.

Abstract

The hybridoma antibody TL5, which precipitates the EGF receptor from the human epidermoid carcinoma cell line A431, has been shown to recognize the blood-group-A carbohydrate structure. This conclusion has been reached from studies of (a) the binding of the antibody to glycoproteins and haemagglutination of erythrocytes with known blood-group-antigen activities and (b) the inhibition of binding of the antibody to a radiolabelled blood-group-A-active glycoprotein by structurally defined oligosaccharides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ABO Blood-Group System / immunology*
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Carcinoma, Squamous Cell / immunology
  • Epitopes / immunology
  • ErbB Receptors
  • Glycoproteins / immunology
  • Hemagglutination Tests
  • Humans
  • Hybridomas / immunology
  • Mice
  • Oligosaccharides / pharmacology
  • Receptors, Cell Surface / immunology*
  • Structure-Activity Relationship

Substances

  • ABO Blood-Group System
  • Antibodies, Monoclonal
  • Epitopes
  • Glycoproteins
  • Oligosaccharides
  • Receptors, Cell Surface
  • ErbB Receptors