A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells

Nature. 1983 Jun;303(5917):497-501. doi: 10.1038/303497a0.

Abstract

The cellular oncogene c-Ki-ras is amplified 30- to 60-fold in cells of the mouse adrenocortical tumour Y1. The amplified oncogene is located in double minute chromosomes and in a homogeneously staining chromosomal region, common karyotypical anomalies of tumour cells. The amounts of c-Ki-ras specific mRNA and of the protein (p21) encoded by the amplified gene are correspondingly elevated. Amplification and enhanced expression of cellular oncogenes may contribute to the genesis and/or maintenance of at least some naturally occurring tumours.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenal Cortex Neoplasms / genetics*
  • Animals
  • Cell Line
  • Chromosomes / physiology
  • DNA Restriction Enzymes
  • Gene Amplification*
  • Karyotyping
  • Mice
  • Neoplasms, Experimental / genetics
  • Nucleic Acid Hybridization
  • Oncogenes*
  • Plasmids
  • RNA, Neoplasm / genetics
  • Transcription, Genetic

Substances

  • RNA, Neoplasm
  • DNA Restriction Enzymes