Abstract
An EcoRI fragment carrying the immunoglobulin C kappa gene and multiple J gene segments from the DNA of the NZB strain of mouse was cloned into lambda Ch 4A DNA. Subsequent characterization of the clone by heteroduplex analysis, restriction-enzyme mapping and DNA sequencing demonstrated that the organization of the J gene segments and the C kappa gene of NZB mouse was similar, if not identical, to that of DNA from the Balb/c strain of mouse. Since the amino acid sequence of the light chain of a plasmacytoma of NZB mouse shows a J region sequence different from that of Balb/c mouse, our results indicate that the new J sequence arose by somatic mutation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacteriophage lambda / genetics
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Base Sequence
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Cloning, Molecular / methods
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Genes
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Immunoglobulin Constant Regions / genetics*
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Immunoglobulin Fragments / genetics
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin kappa-Chains / genetics*
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Immunoglobulins / genetics*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred NZB
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Mutation
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Nucleic Acid Hybridization
Substances
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Immunoglobulin Constant Regions
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Immunoglobulin Fragments
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Immunoglobulin Light Chains
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Immunoglobulin kappa-Chains
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Immunoglobulins
Associated data
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GENBANK/J00549
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GENBANK/J00551
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GENBANK/J00557
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GENBANK/J00558
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GENBANK/J00559
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GENBANK/X00268