Glutathione conjugation of arylnitroso compound: detection and monitoring labile intermediates in situ inside a fast atom bombardment mass spectrometer

Biochem Biophys Res Commun. 1984 Oct 15;124(1):1-5. doi: 10.1016/0006-291x(84)90907-0.

Abstract

The glutathione (GSH) conjugation reaction of the active metabolite of a potent protein-pyrolysate carcinogen, 2-nitroso-6-methyldipyrido[1,2-a:3',2'-d]imidazole (NO-Glu-P-1), occurred in glycerol matrix inside a fast atom bombardment (FAB) mass spectrometer. The short lived GSH-conjugates were detected by in situ FAB analysis. The precursor-product relationship between the conjugates was indicated by following the reaction by measuring [M + H]+ ions of the conjugates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotransformation
  • Carcinogens*
  • Chemical Phenomena
  • Chemistry
  • Glutathione* / metabolism
  • Mass Spectrometry / methods
  • Nitroso Compounds* / metabolism

Substances

  • Carcinogens
  • Nitroso Compounds
  • 2-nitroso-6-methyldipyrido(1,2-a-3',2'-d)imidazole
  • Glutathione