Glutathione-dependent dehydroascorbate reduction: a determinant of dehydroascorbate uptake by human polymorphonuclear leukocytes

Enzyme. 1980;25(3):200-4. doi: 10.1159/000459248.

Abstract

Dehydroascorbate uptake and reduction are impaired in normal human polymorphonuclear leukocytes (PMN) preincubated with N-ethylmaleimide, and in glutathione reductase-deficient cells. We conclude that human PMN dehydroascorbate reduction is glutathione-dependent, and that dehydroascorbate reduction appears to be a necessary condition for cellular dehydroascorbate uptake.

MeSH terms

  • Ascorbic Acid / analogs & derivatives*
  • Dehydroascorbic Acid / metabolism*
  • Glutathione / metabolism
  • Glutathione Reductase / deficiency*
  • Humans
  • Neutrophils / metabolism*
  • Oxidation-Reduction

Substances

  • Glutathione Reductase
  • Glutathione
  • Ascorbic Acid
  • Dehydroascorbic Acid