Isolation of auxotrophs of Bacteroides fragilis

Can J Microbiol. 1978 Dec;24(12):1619-21. doi: 10.1139/m78-260.

Abstract

Techniques employing ethane methanesulfonate and modified penicillin enrichment were developed whereby auxotrophs of Bacteroides fragilis could be readily isolated. Several auxotrophic phenotypes are described.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteroides fragilis / genetics*
  • Bacteroides fragilis / metabolism
  • Ethyl Methanesulfonate
  • Genes
  • Mutation
  • Penicillin G

Substances

  • Ethyl Methanesulfonate
  • Penicillin G