Abstract
Serum samples from several groups of patients attending a gynecology clinic were analyzed by ELISA for specific antibodies recognizing surface epitopes on intact human papillomavirus (HPV) types 6 and 11 L1 virus-like particles (VLPs) that were synthesized in vitro. In these samples, positive IgG and IgM reactivities to HPV-11 L1 VLPs were, respectively, 12% and 6% for 87 controls, 46% and 67% for 79 condyloma patients, 30% and 64% for 72 cervical intraepithelial neoplasia patients, 16% and 19% for 63 pregnant women at time of delivery, and 5% and 0 in their 63 newborns. IgA reactivities were low and not significantly different. The prevalence of IgG-positivity in HPV-6/11 DNA-positive patients increased from 46% with HPV-11 L1 VLPs to 76% when the sera were additionally screened with HPV-6 L1 VLPs. These data show that HPV-6 and -11 L1 VLPs are effective antigens for serologic studies and they detect type-specific antibodies.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Antibodies, Viral / biosynthesis*
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Antibodies, Viral / immunology
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Capsid / immunology*
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Capsid Proteins*
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Case-Control Studies
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Condylomata Acuminata / immunology
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Condylomata Acuminata / virology
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Epitopes
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Female
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Genital Diseases, Female / immunology
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Genital Diseases, Female / virology*
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Humans
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Immunoglobulin A / biosynthesis
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Immunoglobulin G / biosynthesis
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Immunoglobulin M / biosynthesis
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Infant, Newborn
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Oncogene Proteins, Viral / immunology*
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Papillomaviridae / immunology
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Papillomavirus Infections / immunology
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Papillomavirus Infections / virology*
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Pregnancy
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Pregnancy Complications, Infectious / immunology
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Pregnancy Complications, Infectious / virology
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Uterine Cervical Dysplasia / immunology
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Uterine Cervical Dysplasia / virology
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Viral Proteins
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Virion / immunology*
Substances
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Antibodies, Viral
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Capsid Proteins
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Epitopes
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Immunoglobulin A
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Immunoglobulin G
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Immunoglobulin M
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L1 protein, Bovine papillomavirus
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L1 protein, Human papillomavirus type 11
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Oncogene Proteins, Viral
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Viral Proteins
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L1 protein, Human papillomavirus type 6