Abstract
IFN-gamma-secreting T1 and IL-5-secreting T2 subsets of CD8 effectors were generated in vitro using freshly isolated cells from wild-type and perforin knockout mice stimulated with allogeneic Ag-presenting cells. Both T1 and T2 effectors from wild-type mice exhibited perforin-mediated cytolysis. T1, but not T2, populations from perforin knockout mice displayed significant lysis by the Fas-mediated pathway. Th1 cells have recently been shown to be regulated by Fas and we speculate that Fas-mediated mechanisms are involved in the regulation of both Th1 and T1 populations of T cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology
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Antigens, Surface / physiology*
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CD8-Positive T-Lymphocytes / immunology*
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Cytokines / metabolism*
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Cytotoxicity, Immunologic / drug effects
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Cytotoxicity, Immunologic / physiology*
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Egtazic Acid / pharmacology
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H-2 Antigens / immunology
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Lymphocyte Culture Test, Mixed
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Male
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Perforin
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Pore Forming Cytotoxic Proteins
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T-Lymphocyte Subsets / immunology*
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fas Receptor
Substances
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Antigens, Surface
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Cytokines
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H-2 Antigens
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Membrane Glycoproteins
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Pore Forming Cytotoxic Proteins
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fas Receptor
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Perforin
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Egtazic Acid